Fig. 1

Construction of the first set of cotton materials composed of a broad range of MIC value. a F₂ population of the first set. Seven hundred eight F₂ progeny plants were derived from a cross between wild-type (WT) upland cultivar MD52ne and immature fiber (im) mutant. MD52ne produces a phenotype of a fluffy boll, a mature fiber, and a thick wall (inset), whereas the im mutant generates a phenotype of a non-fluffy boll, an immature fiber, and a thin wall (inset). b Genotyping of the F₂ population. Genotypes including homozygosity for the wild type (WT-homo), heterozygosity for the wild type (WT-hetro), and homozygosity for the im type (im-homo) were determined by DNA markers. The WT phenotype (blue) was predicted from the F₂ plants containing WT-homo and WT-hetro genotypes, whereas the im phenotype (red) was expected from the im-homo genotype