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Fig. 1 | Journal of Cotton Research

Fig. 1

From: An isopentyl transferase gene driven by the senescence-inducible SAG12 promoter improves salinity stress tolerance in cotton

Fig. 1

Ectopic-expression of SAG12::IPT promotes cotton seed germination. a Schematic diagram of the SAG12::IPT fragment fused in pSG529 vector. The yellow triangle indicates right boundary (RB). The black rectangle indicates 2.2 kb of Arabidopsis SAG12 promoter fused with BamHI and NcoI. The red rectangle indicates 0.7 kb of Agrobacterium tumefaciens IPT gene fused with NcoI and SacI. The white triangle indicates 0.3 kb of NOS terminator fused with SacI and BamHI. The green triangle indicates NTPII gene generating kanamycin resistance. The blue triangle indicates left boundary (LB). b Ectopic expression of SAG12::IPT promotes cotton seed germination energy. Twenty seeds of each transgenic cotton line were treated with or without 150 and 200 mmol·L− 1 NaCl for 24 h. The germination energy was measured after 3 days NaCl treatment. c Ectopic expression of SAG12::IPT increases cotton seed germination rate. Twenty seeds of each transgenic cotton line were treated with or without 150 and 200 mmol·L− 1 NaCl for 24 h. The germination rate of each line was measured after seven days of NaCl treatment. JIHE321 is the non-transgenic control plant. OE3, OE6, OE7, and OE8 are the transgenic cotton lines with SAG12::IPT. The data are shown as means ± SD from three repeats (n = 3, *P ≤ 0.05)

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