Fig. 3

Ectopic-expression of SAG12::IPT induces cytokinin content in cotton seeds. a Ectopic expression of SAG12::IPT induces Z/ZR-type cytokinin content in seeds of the OE3 and OE8 lines. Germinating seeds (0.5 g, without the seed coat) were sampled after one, two, three, and five days of germination; shoots and roots (0.5 g) separated from the germinating seeds with 220 mmol·L^− 1 NaCl treatment for seven days were collected to detect the Z/ZR-type cytokinin content. b Ectopic expression of SAG12::IPT induces iP/iPA-type cytokinin content in seeds of OE3 and OE8. The samples from (a) were used for the measurement of iP/iPA-type cytokinin. The data are shown as means ± SD from three repeats (n = 3, *P ≤ 0.05)