Fig. 3

Phenotypic observation and expression analysis of GbTRP1-RNAi plants. a Lesion-mimic phenotypes were observed on the stems of GbTRP1-RNAi transgenic lines. Plants were grown in the greenhouse for 2 months and the white arrows indicate the sites of necrosis. b and c RT-qPCR analysis of the expression levels of GbTRP1 and PR genes in WT and GbTRP1-RNAi lines in 10 day-old plants before the lesions appeared on the plants. Error bars represent the standard deviation of the mean of three biological replicates (** p < 0.01, Student’s t-test)