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Fig. 2 | Journal of Cotton Research

Fig. 2

From: Succinate dehydrogenase SDH1–1 positively regulates cotton resistance to Verticillium dahliae through a salicylic acid pathway

Fig. 2

a GhSDH1-1 expression pattern in hormone (SA)-treated ‘Zhongzhimian 2’ plants. a, Treatment with exogenous hormone SA (1 mmol·L-1, 0.1 mmol·L-1, 0.01 mmol·L-1) or an equal amount of sterile water. Mimetics and treated roots were harvested at 1, 6, 12, 24 hpi, respectively, and expression levels were determined by qRT-PCR. The expression level of the simulated control was normalized to ‘1’. Silencing of GhSDH1–1 seriously compromises Verticillium wilt resistance in cotton. b, TRV:PDS as the visual marker for silencing efficiency. Albino phenotype appeared on TRV:SDH1–1 plants. c, Relative transcript levels in control and TRV:SDH1–1 plants 15 d after hand-infiltration. d, Disease symptoms induced with V. dahliae on control and TRV:SDH1–1 plants at 20 dpi and 25 dpi. e, Assessment of DI for TRV:SDH1–1 plants at 20 dpi and 25 dpi. f, Verticillium dahliae recovery assay. g, Vascular browning found in xylem from infected plants at 25 dpi. h, Trypan blue staining. Error bars represent the standard deviation of three biological replicates. Asterisks indicate statistically significant differences as determined by Student’s t test (*P < 0.05; **P < 0.001)

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