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Fig. 2 | Journal of Cotton Research

Fig. 2

From: Overexpressing rice lesion simulating disease 1-like gene (OsLOL1) in Gossypium hirsutum promotes somatic embryogenesis and plant regeneration

Fig. 2

OsLOL1 overexpression represses GhSOD1 expression and activity. a Cell death around embryogenic callus or somatic embryos. Arrows point to the dead cell masses. NP: Calli transformed by empty vector, OE: Calli with OsLOL1 overexpression. b Relative expression level of GhSOD1 in embryogenic calli with empty vector or OsLOL1 gene. GhUbi gene was used as internal control for normalization. The expression levels of OE1 and NP1 are arbitrarily regarded as “1”, respectively. c SOD activity of embryogenic calli with empty vector or OsLOL1 gene. d SOD activity by staining analysis in gel in embryogenic calli with empty vector or OsLOL1 gene. e ROS accumulation of cotton calli by NBT staining analysis. In (d) and (e), NP1 is a represent of the embryogenic calli with empty vector. OE1–3 are representatives of the embryogenic calli with OsLOL1 overexpression. Bar represents SD from three biological repeats in (b) and (c). An asterisk or double asterisks show significant (P < 0.05) or extremely significant (P < 0.01) differences of OEs compared with NP1 by Student’s t-test in (c) and (d)

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