Fig. 4

Expression of GhGT47A1 in Arabidopsis irx10 mutant. a 4-week-old soil-grown wild-type, irx10, GhGT47A1 complemented irx10 plants. b 6-week-old soil-grown wild-type, irx10, GhGT47A1 complemented irx10 plants. C1 represents GhGT47A1 complemented irx10 plants. c RT-PCR analysis of expression of the GhGT47A1, IRX10 in inflorescence stems of wild type, irx10, GhGT47A1 complemented irx10 plants. C1 represents GhGT47A1 complemented irx10 plants. The expression level of ACTIN2 gene was used as an internal control. d Representative image of a branch carrying siliques from different genotypes. C1 represents GhGT47A1 complemented irx10 plants. e-g Toluidine-blue staining of cross sections of roots from wild type (E), irx10 (F), and GhGT47A1 complemented irx10 plants(G). xv denotes xylem vessel, white arrows indicate collapsed xylem vessels. Scale bar = 50 μm. h-j Phloroglucinol staining of cross sections of roots from wild type (H), irx10 (I), and GhGT47A1 complemented irx10 plants (J) . xv denotes xylem vessel, white arrows indicate collapsed xylem vessels. WT represents wild type, irx10 represents irx10 mutant, c1 represents GhGT47A1 complemented irx10 line. Scale bar = 50 μm