Fig. 5

Expression patterns of several GhBLH genes within ovules under GA, BR, and NAA treatment, respectively. a Analysis of GhBLHs with GAREs and P-boxes presented in their promoter regions. b GA activated significant transcription of three GhBLH genes with GAREs and P-boxes in their promoter regions. c Analysis of GhBLHs with CANNT presented in their promoter regions. d BR promoted significant transcription activation of two GhBLH genes with CANNTG. e Analysis of GhBLHs with TAG elements presented in their promoter regions. f NAA failed to activate the transcription of four GhBLH genes with TAG elements. Relative expression levels were determined after normalizing all data to that of CK ovules, which was set to 1.0. Statistical significance was determined using one-way analysis of variance combined with Tukey’s test. *P < 0.05; **P < 0.01; ***P < 0.001. Note: The x-axis represents different hours (6, 12, and 24) after GA, BR, and IAA treatment, the y-axis indicates the relative expression levels. Error bars show the standard deviation of three biological replicates