Fig. 6
PCR analysis of transgene in non-agroinfiltrated leaf samples, DNA isolated from non-infiltrated true leaves. a Lane 1: cotton plant as negative control, lane 2: negative control (TRV1: TRV2 infiltrated), lanes 3 ~ 9: plants infiltrated only with pTH2, lanes 10 ~ 17: plants infiltrated with both pTH2 + pTD2 binary vector, M: 2000 bp marker, H+: positive control for MeHNL gene. b Lane 1: cotton plant as negative control, lane 2: negative control (TRV1: TRV2 infiltrated), lanes 3 ~ 9: plants infiltrated only with pTD2, lanes 10 ~ 17: plants infiltrated with both pTH2 + pTD2 binary vector, D+: positive control for SbDhr2, M: marker (Hind III λ-DNA marker mixed with 2000 bp marker)