Fig. 5

qRT-PCR of GhDAMT and GhALKBH during fiber initiation of Xu142 and Xu142fl. A, B qRT-PCR analysis was performed for four GhDAMT and seven GhALKBH genes responsible for 6mA DNA methylation. Total RNA was extracted from ovules of −2, 0, 5 DPA, and 5 DPA fiber of Xu142 and Xu142fl. All expression levels were normalized to UBQ7 while error bars indicated SD (standard deviation) of three biological replicates. Primers are listed in Additional file 3: Table S2