Fig. 4

Principal components analysis and sample relationship heatmap of RNA-seq data. Samples from M1 (M1_1, M1_2, M1_3), M2 (M2_1, M2_2, M2_3), M3 (M3_1, M3_2, M3_3), M4 (M4_1, M4_2, M4_3) and MA (MA_1, MA_2, MA_3) were clustered into 5 groups, respectively. M1, M2, M3, M4 and MA represent the 1st, 2nd, 3rd, 4th instar nymph and adult, respectively (A). In the heatmap, dark green represents strong correlation, and light green denotes weak correlation. Each column and row correspond to one sample’s relationships with the other 18 samples including itself (B)