From: Genome editing in cotton: challenges and opportunities
Characteristics | TALE | TALEN | References |
---|---|---|---|
Loss-of-function mechanism | Repression of transcription | Frame shift DNA mutation | Shamshirgaran et al. (2022) |
Transgenes | TALE-KRAB | TALEN | Wani et al. (2022) |
Guiding sequence | DBD | DBD | Singh et al. (2022) |
Required sequence information | Annotated TSS | Transcriptome | Anugraha et al. (2022) |
Off-target space | Window around TSS | Genome; requires FokI dimerization | Chaudhuri et al. (2022) |
Transcript variants | Only variants from the same TSS | All variants via conserved region | Li et al. (2022b |