From: Genome editing in cotton: challenges and opportunities
Sr. No | Genome editing tools | Genes | Gene modification | References |
---|---|---|---|---|
1 | Meganucleases | HPPD, EPSPS | HDR, Gene stacking | D'Halluin et al. (2013) |
2 | CRISPR/Cas9 | GhPDS, GhCLA1, GhEF1 | GenEd | Cai et al. (2017) |
3 | CRISPR/Cas9 | GhCLA1, GhVP | NHEJ | Chen et al. (2017) |
4 | CRISPR/Cas9 | GFP | NHEJ | Janga et al. (2017) |
5 | CRISPR/Cas9 | GhCLA1 | Multi-site GenEd | |
6 | CRISPR/Cas9 | GhARG | NHEJ | |
7 | CRISPR/Cas9 | GhMYB25-like A & D | NHEJ | |
8 | CRISPR/Cas9 | GoPGF | NHEJ | Janga et al. (2019) |
9 | CRISPR/Cas | GhCLA, GhPEBP | GenEd | Qin et al. (2020) |
10 | CRISPR/LbCpf1 | GhCLA1 | GenEd | Li et al. (2020) |
11 | CRISPR/Cas12b | GhCLA | GenEd | Wang et al. (2020) |
12 | CRISPR/Cas | Male sterility | NHEJ | Ramadan et al. (2021) |
13 | CRISPR/Cas | MIR482 family | NHEJ | Zhu et al. (2022) |