Fig. 4
Molecular characteristics of the Lu37-1 cotton mutant. a The relative ALS activity of wild-type Lu37 and mutant Lu37-1 in response to different concentrations of imazapic. The absorbance of the sample without imazapic (0 µmol·L−1) was set as 100%, while the others were presented as a percentage value relative to that of 0 µmol·L−1. The values are the means ± SDs of two independent determinations. b Demonstration of the G-to-A mutation at 1 925 bp of the CDS (G1925A, upper case in red) which led to the Ser642Asn mutation in the amino acid sequence of Gh_D10G1253. The primers for the CAPS marker are marked with arrows, and a mismatched base pair (lower case letters in red) was designed in the reverse primer to create a recognition site (CAATTG, marked with the solid box) of endonuclease MfeI. c The illustration of the Ser642Asn (marked by blue line) in the CDS sequnece of Gh_D10G1253. The 1 980-bp Gh_D10G1253 CDS (green arrow) were blasted against the G. hirsutum HAU v1.0 genome on the CottonGen website. The SNPs among 1913 cotton accessions within the region of Ghir_D10: 22854388..22852409 from the results of Li et al. (2021) were marked in red line with corresponding chromosomal site. d Gel image of mutant allele and wild type allele of ALS digested by MfeI. The ALS wild type allele was the 416bp band, while the mutant allele was cleaved into a smaller 383bp band by MfeI