Fig. 5
Overexpression of mutant Gh_D10G1253 conferred herbicide tolerance in Arabidopsis. a At 21 DAT, transgenic Arabidopsis S642N (T3 and T4 generations) cultured on 1/2 MS supplemented with different concentrations of imazapic. Transgenic lines of the T3 generation and the wild-type Columbia (Col) were sown on 1/2 MS supplemented with 50 mg·L−1 kanamycin to confirm the transgenics. b At 21 DAT, transgenic Arabidopsis S642N (T4 generations) sprayed with 2 mL of 0, 1, 4.3, 8.7, 21.8, 44, 109, and 218 µmol·L−1 of imazapic. Col is the wild-type line Columbia, S642N 4-14 and S642N 4-20 are transgenic Arabidopsis S642N lines, and WT 8-2 is a transgenic line with a wild-type allele of ALS gene serving as a transgenic control