Fig. 2

qRT-PCR analysis of RFL gene expression compared with the RNA-seq data from three-line hybrid cotton lines (CMS-D2 line A, maintainer line B, and restoration line R). a The RNA-seq data of RFL genes in A, B and R lines. b The qRT-PCR analysis of three differentially expressed RFL genes. The red columns represented the relative expression levels of the genes; the black lines represented the FPKM number. A: CMS-D2 line, B: maintainer line, R: restorer line