Fig. 4

Phenotypic observation and expression analysis of TRV:TRP1 plants. a Spontaneous lesion formation on the stems and young leaves of TRV:TRP1 cotton seedlings. Images were obtained 21 days after VIGS; Bars = 5 cm. b RT-PCR analysis indicates that the transcripts of GbTRP1 are reduced at 14 days after infiltration (** p < 0.01, Student’s t-test). c RT-qPCR to detect the transcript levels of PR genes in TRV:TRP1 and control plants 14 days after infiltration, before the appearance of lesions. Error bars represent the standard deviation of the mean of three biological replicates(** p < 0.01, Student’s t-test)